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Stability of EPI-X4 JM#21 in human plasma. 20 μmol/L of EPI-X4 JM#21 was diluted in 100% human plasma. The mixture was incubated at 37 °C and samples taken at indicated time points and stored at −80 °C. Samples were thawed in parallel, serially diluted in PBS and activity determined by 12G5-antibody competition. IC50 values were determined by non-linear regression and remaining activity calculated by IC50 (t = 0)/IC50 (t) × 100. Data were represented as mean ± SEM (n = 3).
