Figure 3.

Impact of the time interval between prime and boosting

(A) Two groups of n = 10 BALB/c mice were primed with 1 μg RNA dose of PLX A.I.R/VEEV-gp140 and assigned to immunization schedule 1 (Sch. 1) or schedule 2 (Sch. 2). In schedule 1, 5 mice were euthanized at week 3 (cull 1) and 5 mice were boosted at week 3 and then euthanized at week 5 (cull 2). In schedule 2, 5 mice were euthanized at week 6 (cull 1) and 5 mice were boosted at week 6 and then euthanized at week 5 (cull 2). (B) Antigen-specific IgG titers as determined by ELISA. (C) gp140-specific IFN-γ ELISpots were performed after each euthanasia time point and are reported as spleen forming units (SFU)/10⁶ splenocytes. Box and whiskers, with median, min-max, and 25^th–75^th percentile. Mann-Whitney unpaired t test, p values: ∗<0.05, ∗∗<0.01, ∗∗∗<0.001.