Figure 6.

miR-21 targeted STAT1 to regulate macrophage polarization

(A) Binding sites of the STAT1 3′ UTR to miR-21. (B) Luciferase activity of wild-type (WT) and mutant type (MUT) of STAT1 detected by a luciferase reporter assay. (C) The effect of miR-21 on STAT1 and STAT6 was detected by western blotting. (D) The effect of miR-21 on M1 macrophages (F4/80⁺CD86⁺) and M2 macrophages (F4/80⁺CD206⁺) was detected by flow cytometry. (E and F) The effects of miR-21 on M1 macrophage markers iNOS and CD86 (E) and M2 macrophage markers Arg-1 and IL-10 (F) were detected by quantitative real-time PCR. Data represent the mean ± SD of three experiments performed in triplicate independently. ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001.