REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
Mouse anti-CD90.2 (53-2.1)	Ebioscience	Cat#47-0902-82; RRID: AB_1272187
Mouse: anti-F480 (BM8)	Ebioscience	Cat#47-4801-80; RRID: AB_2637188
Mouse: anti-Gr1(RB6-8C5)	Ebioscience	Cat#47-5931-80; RRID: AB_1518805
Mouse: anti-B220 (RA3-6B2)	BD	Cat#563793; RRID: AB_2738427
Mouse: anti-IgM (11/41)	Ebioscience	Cat# 25-5790-82; RRID: AB_469655
Mouse: anti-IgD (11-26c.2a)	BD	Cat#563618; RRID: AB_2738322
Human: anti-CD3 (OKT3)	Ebioscience	Cat#47-0037-42; RRID: AB_2573936
Human: anti-CD14 (61D3)	Ebioscience	Cat#47-0149-42; RRID: AB_1834358
Human: anti-CD16 (CB16)	Ebioscience	Cat#47-0168-42; RRID: AB_11220086
Human: anti-CD19 (HIB19)	Biolegend	Cat#302242; RRID: AB_2561381
Human: anti-CD20 (2H7)	BD	Cat#563782; RRID: AB_2744325
Human: anti-CD21 (B-ly4)	BD	Cat#562966; RRID: AB_2737921
Human: anti-CD27 (O323)	Biolegend	Cat#302834; RRID: AB_1121920
Human: anti-CD38 (HB-7),	Biolegend	Cat#356620; RRID: AB_2566232
Human: anti-CD11c (B-ly6)	BD	Cat#562393; RRID: AB_11153662
Human: anti-CD79b (CB3-1)	Biolegend	Cat#341404; RRID: AB_1595454
Human: anti-IgD (IA6-2)	Biolegend	Cat#348210; RRID: AB_10683460
Human: anti-IgM (MHM-88)	Biolegend	Cat#314524; RRID: AB_2562373
Human: anti-IgA	Southern Biotech	Cat#2050-02; RRID: AB_2795702
Human: anti-IgG (G18-145)	BD	Cat#564230; RRID: AB_2738684
Human: Fc block	BD	Cat#564219; RRID: AB_2728082
Mouse: Fc block CD16/CD32	BD	Cat# 553141; RRID: AB_394656
Human: HRP-labeled anti-Ig heavy and light chain	Invitrogen	Cat#31412; RRID: AB_228265
Biological samples
Human blood samples	University of Minnesota	N/A
Chemicals, peptides, and recombinant proteins
Ghost Red 710 viability dye	TonBo	Cat#13-0871-T100
Collagenase D	Roche/Boehringer	Cat# 1088874
S1-RBD/SA-AF647 tetramer	This paper	N/A
decoy SA-PE-AF647	This paper	N/A
AccuCheck Counting Beads	ThermoFisher	Cat#PCB100
KPL ABTS® Peroxidase Substrate (1-Component)	Sera Care	Cat#5120-0043
anti-Cy5/AF647 MicroBeads	Miltenyi	Cat#130-091-395
BD Cytofix/Cytoperm	BD Biosciences	Cat#51-2090KZ
Flow Cytometry Perm Buffer	TONBO	Cat#TNB-1213-L150
Complete Freund’s Adjuvant (CFA)	Sigma	Cat#F5881
10K Amicon Ultra Centrifugal Filters	Millipore	Cat#UFC801008
100K Amicon Ultra Centrifugal Filters	Millipore	Cat#UFC510008
SA-PE Phycolink	Prozyme	Cat#PJRS27
SA-AF647	ThermoFisher	Cat#S21374
Critical commercial assays
Effectene kit	QIAGEN	Cat#301425
Bir A protein ligase kit	Avidity	Cat#BirA500
Experimental models: Cell lines
HEK293T cells	ATCC	Cat#CRL-3216; RRID: CVCL_0063
Experimental models: Organisms/strains
Mouse: C57BL/6NCr (B6)	Charles River/NCI	Cat#556
Oligonucleotides
T7-Promoter (5′-TAATACGACTCACTATAGGG-3′)	This paper	N/A
BGH-reverse (5′-TAGAAGGCACAGTCGAGG-3′)	This paper	N/A
Recombinant DNA
gene block encoding S1-RBD	This paper	N/A
pcDNA3.1/Zeo (+) Mammalian Expression Vector	ThermoFisher	Cat #: V86020
gene block encoding the BirA sequence	This paper	N/A
Software and algorithms
FlowJo software	Tree Star	https://www.flowjo.com
Prism software	GraphPad	https://www.graphpad.com/scientific-software/prism/
Other
CPT Mononuclear Cell Preparation Tubes-sodium citrate	BD	Cat#362761