Figure 6.

CDC42 knockdown represses the malignant phenotypes of GC cells via regulating ITGB1/FAK/PXN/AKT signaling

(A) The knockdown of CDC42 in HGC-27 (upper) and AGS (lower) cells after CDC42 siRNA transfection was confirmed by qPCR. (B−E) After transfection, the colony-formation ability (B), proliferation (C), apoptosis (D), and migration and invasion (E) of GC cells were examined by plate colony-formation assay, EdU assay, flow cytometry analysis, and Transwell assay, respectively. (F) After transfection, western blotting was used to detect the expressions of ITGB1, phospho-focal adhesion kinase (pFAK) (Tyr397), total FAK, pPXN (Tyr118), total PXN, pAKT (Ser473), total AKT, and CDC42 in GC cells (scale bars, 50 μm). ∗p < 0.05; ∗∗p < 0.01; ∗∗∗p < 0.001.