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| Reagent | Initial concentration | Final concentration | Volume (μL) per reaction | Volume (μL) for 3 reactions |
|---|---|---|---|---|
| 5× Reaction buffer | 5× | 1× | 8 | 24 |
| NTP mix | 25 mM | 10 mM | 16 | 48 |
| Enzyme mix | - | - | 4 | 12 |
| Nuclease-free water a | - | - | 11 | 33 |
| Final volume | 39 | 117 |
