4× RPB Buffer

Stocks	Final concentration	Amount
5× RPB Buffer	4×	200 μL
10 mM dNTPs mixture	1.6 mM	40 μL
Nuclease-free water	n/a	10 μL
Total	n/a	250 μL

∗Storage at – 20°C. Each 250 μL aliquot yields 50 RT-PCR reactions.