Figure 2.

IRF5 silencing effect of biodegradable LNPs in macrophages

IRF5 expression analysis following intravenous injection of siIrf5-LNPs in mice. Mice were injected with siIrf5-LNPs or siCtrl-LNPs at a siRNA dose of 5.0 mg/kg. (A) Twenty-four hours after LNP administration, Irf5 mRNA expression in Mϕs and B cells in the spleen, PECs, and liver were analyzed by qRT-PCR. Values were normalized to Gapdh levels using the ΔΔCt method. (B) After LNP administration, IRF5 protein expression in liver Mϕs and B cells was analyzed by immunofluorescent staining at indicated time points. Boxplots indicate MFI of IRF5 expression. Data from three independent experiments are shown. ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001 (Student’s t test). PEC, peritoneal exudate cell. See also Figures S4, S5, and S6.