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. Author manuscript; available in PMC: 2022 Oct 1.
Published in final edited form as: Gastroenterology. 2021 Jun 9;161(4):1303–1317.e3. doi: 10.1053/j.gastro.2021.05.060

Figure 5.

Figure 5.

CircHIPK3 acts as a sponge of miR-29b in vitro. (A) Schematic of the putative binding sites of miR-29b on circHIPK3 transcript. (B) Levels of miR-29b 24 h after transfection with biotinylated miR-29b. Values are the means ± SEM (n = 3). * P <0.05 compared with control scramble oligomer. (C) Binding of biotinylated miR-29b to various circRNAs. Left, levels of circHIPK3, Cdr1as, and circPABPN1 in the materials pulled down by biotin-miR-29b; and right, levels of total input circRNAs. (D) Levels of luciferase reporter activity after circHIPK3 silencing or its overexpression. Left: Schematic of the chimeric mRNA vector bearing two perfect binding sites (BS) of miR-29b. * P <0.05 compared with cells transfected with C-siRNA or control vector.