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. Author manuscript; available in PMC: 2022 Oct 1.
Published in final edited form as: Gastroenterology. 2021 Jun 9;161(4):1303–1317.e3. doi: 10.1053/j.gastro.2021.05.060

Figure 6.

Figure 6.

CircHIPK3 regulates intestinal epithelial repair by interacting with miR-29b. (A) Levels of miR-29b (left) and U6 RNA (right) 24 h after transfection with biotinylated miR-29b. Values are the means ± SEM (n = 3). * P <0.05 compared with control scramble. (B) Levels of mRNAs encoding Rac1, Cdc42, RhoA, cyclin B1 (CycB1), CDK4, and p21 in the materials pulled down by biotin-miR-29b. * P <0.05 compared with control scramble. (C) Immunoblots of various proteins. (D) Immunostaining of F-actin at 6 (top) and 24 (bottom) h after wounding in cells described in C. (E) Summarized data in cells described D (bottom). *, +P < 0.05 compared with control and miR-29b expression alone, respectively. (F) Model proposed to explain the influence of circHIPK3 upon intestinal epithelial repair after wounding.