FIG. 8.

SP600125‐mediated removal of ph‐S6‐p53/PARP1 complexes from ALCDs inhibits proliferation of HBL cells. (A) ChIP assay shows that the treatments of Huh6 and HepG2 cells with SP600125 remove the ph‐S6‐p53/PARP1 complexes from ALCDs of NRF2 and beta‐catenin genes. “Inactive” ALCD within the p21 gene was included as the control. (B,C) Inhibition of proliferation of Huh6 and HepG2 cells by Ola and SP600125 and by combination of these drugs. Images of untreated HepG2 and Huh6 cells and cells treated with drugs, as shown on the right. (D) WB of the cell cycle proteins using protein lysates from untreated and treated Huh6 and HepG2 cells. (E) Hypothesis showing mechanisms by which Ola inhibits pediatric liver cancer.