Key Resource Table
| Resource Type | Specific Reagent or Resource | Source or Reference | Identifiers | Additional Information |
|---|---|---|---|---|
| Add additional rows as needed for each resource type | Include species and sex when applicable. | Include name of manufacturer, company, repository, individual, or research lab. Include PMID or DOI for references; use “this paper” if new. | Include catalog numbers, stock numbers, database IDs or accession numbers, and/or RRIDs. RRIDs are highly encouraged; search for RRIDs at https://scicrunch.org/resources. | Include any additional information or notes if necessary. |
| Antibody | ||||
| Polyclonal rabbit anti-cFos | Millipore Sigma | F7799 | ||
| Goat Anti-Rabbit IgG AlexaFluor-488-conjugated | Jackson ImmunoResearch Laboratories Inc. | 111-545-144 | ||
| Bacterial or Viral Strain | ||||
| AAV2-EF1a-DIO-hChR2(H134R)-mCherry-WPRE-pA | University of North Carolina Vector Core | N/A | ||
| AAV2-EF1a-DIO-NpHR3.0-mCherry | University of North Carolina Vector Core | N/A | ||
| Chemical Compound or Drug | ||||
| 95% Ethyl Alcohol (ACS/USP Grade) | PHARMCO-AAPER | 111000190 | ||
| Ketamine | VedCo | CAS: 6740-88-1 | KETAVED (KETAMINE HCL 100MG) | |
| Xylazine | Patterson Veterinary | CAS: 7361-61-7 | AnaSed | |
| Carprofen | Patterson Veterinary | CAS: 53716-49-7 | Rimadyl Injectable | |
| Saline | Hospira | 00409-4888-12 | ||
| 4% Paraformaldehyde in 1xPBS | Alfa Aesar | CAS: 30525-89-4 | ||
| Phosphate buffered saline (10x) | Fisher Scientific | BP3991 | ||
| Triton X-100 | Millipore Sigma | CAS: 9002-93-1 | ||
| DAPI | Millipore Sigma | CAS: 28718-90-3 | ||
| Krystalon | Millipore Sigma | 64969 | ||
| Cresyl violet | Millipore Sigma | C5042 CAS: 10510-54-0 | ||
| Commercial Assay Or Kit | ||||
| Alcohol analyzer | Analox | AM1 | ||
| Alcohol analyzer reagent | Analox | GMRD-113 | ||
| Alcohol analyzer standard | Analox | GMRD-110(100) | ||
| Organism/Strain | ||||
| Swiss webster (CFW) mice (male and female) | Charles River Laboratories | 024 | Males castrated by Charles River Laboratories | |
| C57BL/6J mice (female) | The Jackson Laboratory | 000664 | ||
| B6.Cg-Gt(ROSA)26Sor tm9(CAG-tdTomato)Hze/J (Ai9) mice | The Jackson Laboratory | 007909 | Bred to CRH-ires-Cre mice in-house | |
| B6(Cg)-Crh tm1(cre)Zjh/J (CRH-ires-Cre) mice | The Jackson Laboratory | 012704 | Experimental mice bred in-house | |
| Software; Algorithm | ||||
| GraphPad Prism | GraphPad Software, Inc | v. 8.0 and v. 9.0 | ||
| The Observer XT | Noldus Information Technology | v. 12.0 | ||
| FIJI ImageJ | Schindelin J, Arganda-Carreras I, Frise E et al. (2012): Fiji: an open-source platform for biological-image analysis. Nat Methods 9: 676-682, PMID 22743772, doi:10.1038/nmeth.2019 | v. 1.53e | https://imagej.net/Fiji | |
| ZEN Lite | Zeiss | N/A | https://www.zeiss.com/microscopy/us/products/microscope-software/zen-lite.html | |
| MATLAB | Mathworks | R2019b | ||
| Med-PC | Med Associates Inc | IV | ||
| Biorender | Biorender | N/A | Biorender.com | |
| Other | ||||
| Optic fiber implants | Doric lenses | MFC_200/240-0.22_5mm_ZF1.25_FLT | ||
| Mating sleeve | Doric lenses | SLEEVE_ZR_1.25_BK | ||
| Patch cords | Doric lenses | MFP FC-FC and FC-ZF1.25 | ||
| Rotary joint | Doric lenses | FRJ_1×1_FC | ||
| Gimbal | Doric lenses | GH_FRJ | ||
| Stereotaxic frame | Kopf | Model 902 | ||
| Stereotaxic syringe pump | World Precision Instruments | UMP3T-2 | ||
| Sub-microliter syringe | World Precision Instruments | NANOFIL (10) | ||
| Microinfusion needles (beveled) | World Precision Instruments | NF35BV-2 | ||
| Submandibular mouse lancet | MEDIpoint Inc. | Goldenrod 3 mm | ||
| Dental cement | Stoelting Co. | 51459 | ||
| Lasers | CrystaLaser | 473 nm, 561 nm | ||
| Pulse generator | A.M.P.I. | Master-8 | ||
| Lickometer insert | Med Associates | ENV-350RM | ||
| Lickometer controller | Med Associates | ENV-250B | ||
| Interface module, connection panel | Med Associates | DIG-716B, SG-716 | ||
| Home cage lickometer panel | Machined in-house | N/A | ||
| Video camera | Logitech | C920 | ||
| Microscope | Zeiss | AxioImager Z1 | ||
| Microscope camera | Zeiss | AxioCam Mrm v.3.0 | ||
| Cryostat | Leica | CM3050 | ||