Fig. 3.

Evolutionary history of Cucurbita NLR gene family. a Phylogenetic tree of Cucurbita NLR gene family identified in four genome assemblies. Clades are collapsed based on a bootstrap value over 80 and numerated. b NLR gene family expansions and contractions along dated phylogeny of the Cucurbita genus. The tart charts and the numbers at every branch of the tree indicate whether a gene family expanded (blue), contracted (red), or remained the same size (gray). The gene birth/death rate (λ) in the most recent common ancestor of the phylogeny and the whole-genome duplication (yellow star) in Cucurbita sp. were indicated