Apoptosis in hepatic tissues was determined by TUNEL assays, caspase 3 activity assays, and Western blotting, and the results show the expression of apoptosis-associated proteins. a Representative TUNEL staining images for each group (original magnification, ×400). b The number of TUNEL-positive cells in each group. c Caspase 3 activity. d Bcl-2 expression. e Bax expression. Mean ± SEM, n = 6–8 mice in each group; *P < 0.05 between the indicated groups.