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. 2021 Sep 24;10:197. doi: 10.1038/s41377-021-00628-0

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 7 — The ×5 microscopic imaging of cerebral vasculature in the same mouse in (a) 1400–1550 nm, (b) 1425–1475 nm, (c) 1500–1700 nm, and (d) 1550–1700 nm. Scale bar: 300 μm. e–h Cross-sectional fluorescence intensity profiles along the indigo lines of the blood vessel in (a–d). The numbers show the SBRs. The ×25 microscopic imaging of cerebral vasculature in the same mouse at depth of 150 μm in (i) 1400–1550 nm and (j) 1550–1700 nm. Scale bar: 100 μm. The ×25 microscopic imaging of cerebral vasculature in the same mouse at depth of 650 μm in (k) 1400–1550 nm and (l) 1550–1700 nm. Scale bar: 100 μm. Cross-sectional fluorescence intensity profiles along (m, n) the brown lines in (i, j) and (o, p) the indigo lines in (k, l). The numbers show the SBRs. q ×25 microscopic imaging of cerebral vasculature in 1400–1550 nm at various depths below the skull and the fluorescence intensity analysis of the blood vessel at the depth of 900 μm (the brown line). The brown arrows show the deep and tiny capillaries. Scale bar: 100 μm.

Fig. 7 — The ×5 microscopic imaging of cerebral vasculature in the same mouse in (a) 1400–1550 nm, (b) 1425–1475 nm, (c) 1500–1700 nm, and (d) 1550–1700 nm. Scale bar: 300 μm. e–h Cross-sectional fluorescence intensity profiles along the indigo lines of the blood vessel in (a–d). The numbers show the SBRs. The ×25 microscopic imaging of cerebral vasculature in the same mouse at depth of 150 μm in (i) 1400–1550 nm and (j) 1550–1700 nm. Scale bar: 100 μm. The ×25 microscopic imaging of cerebral vasculature in the same mouse at depth of 650 μm in (k) 1400–1550 nm and (l) 1550–1700 nm. Scale bar: 100 μm. Cross-sectional fluorescence intensity profiles along (m, n) the brown lines in (i, j) and (o, p) the indigo lines in (k, l). The numbers show the SBRs. q ×25 microscopic imaging of cerebral vasculature in 1400–1550 nm at various depths below the skull and the fluorescence intensity analysis of the blood vessel at the depth of 900 μm (the brown line). The brown arrows show the deep and tiny capillaries. Scale bar: 100 μm.