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. 2021 Sep 24;11:19011. doi: 10.1038/s41598-021-97866-4

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Comparison of streptolysin production and activity in emm type 1.0 grown in vitro or recovered from in vivo. (A) Concentration of streptolysin (ng/ml) secreted into the supernatant by emm type 1.0 (isolate 101,910) grown in vitro or recovered from knee joints (P101910) and then grown in vitro. (B) SLO haemolytic activity. (C) After subsequent in vitro passaging of in vivo recovered P101910 in Todd-Hewitt broth, the concentration of streptolysin (ng/ml) was measured, (D) and the SLO haemolytic activity. **p value < 0.01, ***p < 0.005 and ****p value < 0.001 two-way ANOVA followed by a Bonferroni’s multiple comparisons correction.