Table 1.

Plant growth regulator (PGR) treatments and concentrations and supplement contents were applied by somatic embryogenesis. Note that the base medium was half-strength Litvay’s medium.

Step	PGR concentration (μM)	Supplement (g/L)
Initiation	Low: BA 1.25 + 2,4-D 2.5 BA 2.5 + 2,4-D 5.0 Standard: BA 5.0 + 2,4-D 10.0 High: BA 7.5 + 2,4-D 12.5 BA 7.5 + 2,4-D 15.0 BA 10.0 + 2,4-D 15.0 BA 10.0 + 2,4-D 20.0	Sucrose 10.0 Gelrite 4.0 Glutamine 0.5
Proliferation	Liquid: BA 3.75 + 2,4-D 6.25	Sucrose 10.0 Glutamine 0.5
	Solid: BA 7.5 + 2,4-D 12.5	Sucrose 10.0 Gelrite 4.0 Glutamine 0.5
Maturation	ABA 60.0	Sucrose 60.0 Gelrite 6.0 Glutamine 0.5
Germinationa		Sucrose 20.0 Gelrite 6.0 Glutamine 0.5

^aContents for all half-strength Litvay’s medium elements were reduced to half other than KNO₃, KH₂PO₄, and Fe-EDTA.