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. 2021 Jan 25;42(10):1587–1597. doi: 10.1038/s41401-020-00585-1

Fig. 3. Zonisamide inhibited cardiac hypertrophy and fibrosis in vitro.

Fig. 3

NRCMs and cardiac fibroblasts were incubated with zonisamide (0.1, 0.3, 1 μM dissolved in 0.1% DMSO) and Ang II for 24 h. a mRNA levels of ANF, β-MHC, and α-MHC in NRCMs and Collagen 1A1 in cardiac fibroblasts were assessed by RT-PCR (n = 5). b NRCMs were stained with phalloidin (phalloidin-tetramethylrhodamine isothiocyanate) and DAPI to evaluate cell surface area. c Quantitative analysis of cell surface area with ImageJ software (n = 4) (scale = 50 µm). Data were analyzed by one-way ANOVA. Values are presented as the mean ± SEM. *P < 0.05, **P < 0.01 vs. Con; #P < 0.05, ##P < 0.01 vs. Ang II.