Skip to main content
. 2021 Sep 24;11(10):448. doi: 10.1007/s13205-021-02990-7

Table 2.

Primers and their sequences used in this work

Primer Function Sequence 5ʹ → 3ʹ
P43-F1 Amplification of P43 CCCAAGCTTGCGGCTTCCTTGTAGAGCTCAGC (HindIII)
P43-R1 CGCGGATCCCTCGAGACGCGTGCATGCTCTAGAAGATCTCTGCAGGTCGACCATGTGTACATTCCTCTC (BamHI)
sfp-F Amplification of sfp GCGTCGACCCAAGGAGGGTATAGCTATGAAAATTTACGGAGTATATATG (SalI)
sfp-R GAAGATCTTTATAACAGCTCTTCATACGTTTTC (BglII)
degQ-F Amplification of degQ GAAGATCTCCAAGGAGGGTATAGCTATGGAAAAGAAACTTGAAGAAG (BglII
degQ-R CGGAATTCTTTCTCCTTGATCCGGACAGAATC (EcoRI)
P43-F2 Amplification of P43 CCAAGCTTTGATAGGTGGTATGTTTTCGCT (HindIII)
P43-R2 CGGATCCCAGTCTAGACACCTCGAGGCGAGATCTCATGTGTACATTC (BamHI)
accA-F Amplification of accA GAAGATCTCCAAGGAGGGTATAGCTGTGGCTCCAAGATTAGAATTTG (BglII)
accA-R CCGCTCGAGTTAGTTTACCCCGATATATTG (XhoI)
cypC-F Amplification of cypC CGCTCGAGCCAAGGAGGGTATAGCTATGAATGAGCAGATTCCACATG (XhoI)
cypC-R GCTCTAGATTAACTTTTTCGTCTGATTCCG (XbaI)
gapA-F Amplification of gapA GCTCTAGACCAAGGAGGGTATAGCTATGGCAGTAAAAGTCGGTATT (XbaI)
gapA-R CGGGATCCTTAAAGACCTTTTTTTGCGATG (BamHI)
pHY-F Sequencing of overexpressed strains GGCAGGAACAGGAGAGCGCACGAG
pHY-R GGTTGTTACCTATGGAAGTTGATCAGTC

Underlined parts: the restriction enzyme cutting site