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. 2021 Sep 24;11(10):448. doi: 10.1007/s13205-021-02990-7

Table 3.

Enzyme-specific activity of parent strain B. subtilis 168 and recombinants in batch cultures

Strain Time (h) Enzyme activities (U/mg protein)
Acetyl-CoA carboxylase Fatty acid beta-hydroxylating cytochrome P450 Glyceraldehyde-3-phosphate dehydrogenase
B. subtilis 168 24 0.18 ± 0.03 0.23 ± 0.03 0.31 ± 0.03
48 0.15 ± 0.02 0.21 ± 0.03 0.27 ± 0.02
BSP00A 24 0.41 ± 0.03 0.24 ± 0.04 0.30 ± 0.04
48 0.38 ± 0.02 0.21 ± 0.03 0.28 ± 0.02
BSP00C 24 0.19 ± 0.03 0.42 ± 0.03 0.31 ± 0.03
48 0.16 ± 0.02 0.40 ± 0.03 0.26 ± 0.03
BSP00G 24 0.18 ± 0.03 0.23 ± 0.03 0.58 ± 0.05
48 0.15 ± 0.02 0.22 ± 0.02 0.53 ± 0.03
BSP002 24 0.42 ± 0.03 0.41 ± 0.03 0.32 ± 0.03
48 0.38 ± 0.02 0.40 ± 0.03 0.28 ± 0.04
BSP003 24 0.42 ± 0.03 0.43 ± 0.03 0.59 ± 0.05
48 0.40 ± 0.02 0.41 ± 0.03 0.55 ± 0.04

Results are represented as mean ± SD of three independent observations