Table 3.
Enzyme-specific activity of parent strain B. subtilis 168 and recombinants in batch cultures
| Strain | Time (h) | Enzyme activities (U/mg protein) | ||
|---|---|---|---|---|
| Acetyl-CoA carboxylase | Fatty acid beta-hydroxylating cytochrome P450 | Glyceraldehyde-3-phosphate dehydrogenase | ||
| B. subtilis 168 | 24 | 0.18 ± 0.03 | 0.23 ± 0.03 | 0.31 ± 0.03 |
| 48 | 0.15 ± 0.02 | 0.21 ± 0.03 | 0.27 ± 0.02 | |
| BSP00A | 24 | 0.41 ± 0.03 | 0.24 ± 0.04 | 0.30 ± 0.04 |
| 48 | 0.38 ± 0.02 | 0.21 ± 0.03 | 0.28 ± 0.02 | |
| BSP00C | 24 | 0.19 ± 0.03 | 0.42 ± 0.03 | 0.31 ± 0.03 |
| 48 | 0.16 ± 0.02 | 0.40 ± 0.03 | 0.26 ± 0.03 | |
| BSP00G | 24 | 0.18 ± 0.03 | 0.23 ± 0.03 | 0.58 ± 0.05 |
| 48 | 0.15 ± 0.02 | 0.22 ± 0.02 | 0.53 ± 0.03 | |
| BSP002 | 24 | 0.42 ± 0.03 | 0.41 ± 0.03 | 0.32 ± 0.03 |
| 48 | 0.38 ± 0.02 | 0.40 ± 0.03 | 0.28 ± 0.04 | |
| BSP003 | 24 | 0.42 ± 0.03 | 0.43 ± 0.03 | 0.59 ± 0.05 |
| 48 | 0.40 ± 0.02 | 0.41 ± 0.03 | 0.55 ± 0.04 | |
Results are represented as mean ± SD of three independent observations