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. 2021 Jan 29;42(10):1676–1689. doi: 10.1038/s41401-020-00575-3

Fig. 7. MiR-708 inhibited tumor growth in nude mice of HCC model.

Fig. 7

MiR-708 inhibited tumor growth in nude mice of HCC model. a A stable SMMC-7721 line was established by using LV-miR-708, with LV-miR-NC used as a negative control. Then, the HCC model was established by subcutaneous injection of nude mice of LV-miR-708 or control LV-miR-NC. b Stable control and LV-miR-708-transfected SMMC-7721 cell lines were observed by inversed fluorescent microscope. c, e Tumor images and weights at experimental endpoints in NC and LV-miR-708-transfected SMMC-7721 cells xenografts (n = 6 for each group). d The H&E stain in the LV-miR-NC group and LV-miR-708 group. IHC results of E-cadherin, N-cadherin, and Vimentin were shown in the LV-miR-NC group and LV-miR-708 group. f The protein expression of ZEB1 in the LV-miR-NC group and LV-miR-708 group. g Western Blotting analysis of E-cadherin, N-cadherin, and Vimentin in tumor tissues of LV-miR-NC, and LV-miR-708 groups. h Western blotting analysis of MMP9, MMP2, PCNA, Bax and Bcl-2 in tumor tissues of LV-miR-NC and LV-miR-708 groups. Data are presented as the mean ± SD of three independent experiments. *P < 0.05, **P < 0.01, compared to the control group.