Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2021 Sep 24;12(10):871. doi: 10.1038/s41419-021-04159-9

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2021, corrected publication 2024

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Fig. 2 — A Immunoblot analysis of NLRP3 and IL-33 protein expression in HaCaT cells in response to LPS (10 μg/mL, 24 h), ATP (5 mM, 1 h), or LPS (10 μg/mL, 24 h) and ATP (5 mM, 1 h) stimulation. B Relative expression of Nlrp3 and Il33 mRNA in HaCaT cells in response to LPS (10 μg/mL, 24 h) and ATP (5 mM, 1 h) stimulation by qRT-PCR analysis. C ELISA analysis of IL-33 secretion level from HaCaT cells in response to LPS (10 μg/mL, 24 h) and ATP (5 mM, 1 h) stimulation. D–F, Immunoblot analysis of NLRP3 and IL-33 protein expression in HaCaT cells following overexpression of NLRP3 by transfecting with pLV-EGFP-NLRP3, or interfering of NLRP3 or IL-33 with specific siRNAs. G qRT-PCR analysis of Il1β and Il18 mRNA expression in HaCaT cells upon LPS (10 μg/mL, 24 h) and ATP (5 mM, 1 h) stimulation. H ELISA analysis of the secretion of IL-1β and IL-18 from HaCaT cells in response to LPS (10 μg/mL, 24 h) and ATP (5 mM, 1 h) stimulation. Statistical comparisons were performed using unpaired two-tailed Student’s t test (all data are represented as the mean ± SD of three independent experiments, *P < 0.05; **P < 0.01 vs. Control).