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. 2021 Sep 24;11:19049. doi: 10.1038/s41598-021-98566-9

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Glucose-injected larvae have increased lipid accumulation following a high fat diet. (A) Schematic of the high fat feeding challenge assay to measure lipid accumulation in glucose-injected larvae. (B) Bright field images of 6 dpf Oil Red O-stained larvae, demonstrating darker vascular staining in glucose-injected larvae. Box indicates location of inset, arrowheads indicate stained intersegmental vessels in inset, asterisk indicates intestinal lumen which was excluded from analysis. (C) Quantification of lipid accumulation in 5 dpf glucose-injected larvae. (D) Quantification of lipid accumulation in 6 dpf glucose-injected larvae challenged with a high fat diet from 5 dpf. (E) Quantification of lipid accumulation in 7 dpf glucose-injected larvae challenged with a high fat diet from 5 dpf. Statistical testing by t test. Data are representative of 2 biological replicates.