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. 2021 Sep 24;11:19063. doi: 10.1038/s41598-021-98600-w

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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TACS-2 and -3 are both present in this figure. These collagen fibers are easily identifiable in the PPM image (right) by a color contrast compared to other tissue parts, the highly aligned fibers are also seen with similar hue (purple) that are perpendicular to tumor boundary in TACS-3 region and tangential to tumor boundary in TACS-2 regions, while none of these information can be readily extracted from the BF image only. The tissue area represented in images is 692 × 519 µm.