Fig. 2.

ADAM10 controls the terminal differentiation of splenic Langerin⁺ cDC1. (A) Frequency of Langerin⁺CD103⁺ cells within the XCR1⁺ cDC1 cDC population in spleens of control and ADAM10^ΔCD11c mice. (B) Absolute numbers of splenic Langerin⁺ and Langerin^– cDC1 in control and ADAM10^ΔCD11c mice. (C) Frequency of ESAM^hi cells within the Langerin⁺ cDC1 subset. (D) Expression of CD8-α and CD24 on splenic cDC1 isolated from control and ADAM10^ΔCD11c mice, assessed by flow cytometry gated on CD11c⁺MHCII⁺XCR1⁺ cells. Filled histogram: isotype control. (E) Frequency of in vivo CD11c-labeled cDC1 in spleens of control and ADAM10^ΔCD11c mice. (F) Representative flow cytometry plot showing the frequency of splenic Langerin⁺XCR1⁺ cDC1 in control (Top), ADAM10^ΔXCR1 (Middle), and ADAM10^ΔCX3CR1 (Bottom) mice. Bar graph indicates the average frequency of these cells found in the spleen of the indicated mice. (G) Volcano plot representing the gene expression changes in splenic cDC1 from ADAM10^ΔCD11c/control mice (P value versus log₂ fold change). Genes also significantly changed in CD103⁺ versus CD103^– cDC1 are indicated in red (published dataset) (11). *P < 0.05, **P < 0.01, and ***P < 0.001 (Student’s t test or one-way ANOVA). FACS plots show one representative mouse/group with mean frequencies or mean fluorescent intensities ± SEM. Data are pooled from at least three experiments (n = 4 to 6 mice/experiment). SC-seq-WTA is a visualization of three Cre-negative and three Cre-positive ADAM10^ΔCD11c mice.