Fig. 7.

Doxycycline does not affect the protease resistance of mutant PrP in the brains of Tg(FFI-26) mice. (A) Brain lysates from mice sacrificed at the end of the treatment (249 ± 9 days of age) (200 μg of total proteins) were incubated with 0–2 μg/mL PK for 1 h at 4 °C, and PrP was visualized by western blotting using monoclonal antibody 6D11. Each sample represents 100 μg of protein. (B) The percentage of PrP remaining after PK digestion was quantified by densitometric analysis of western blots. Each point represents the mean ± SEM of 3–4 replicates.