Figure 4.

Full-length extension by hpol η and hpol κ in the presence of all four dNTPs.A, 14-mer primer and 19-mer template DNA sequences, where X is dG, N²-dG-15-mer peptide cross-link, or N²-dG-15-mer peptide cross-link. Reactions were done in the presence of: B, 20 nM hpol η; C, 20 nM hpol κ. All reactions were done at 37 °C for 0, 5, 10-, 30-, 60-, or 120-min. Lanes 1–6 have X as dG, lanes 7–12 have X as N²-dG-15-mer peptide cross-link, and lanes 13–18 have X as N²-dG-36-mer peptide cross-link. P, FAM-labeled 14-mer DNA primer.