Table 2.
Summary of products of extension of template–primer complexes by hpol η analyzed by LC-ESI-MS/MS
| Primer: 5′-FAM-GGTGGTCCAUAAAC-3′ Template: 3′-CCACCAGGTATTTGXCTCT-5′ | ||||
|---|---|---|---|---|
| X | Sequence | Yield (%) | Observed m/z (charge) | Base added |
| dG | 5′-pAAACCGAGA-3′ | 25% | 939.36 (−3) | C |
| 5′-pAAACCGAGAA-3′ | 71% | 1043.64 (−3) | C, blunt end addition of A | |
| 5′-pAAACCGAGAAA-3′ | 4% | 1148.00 (−3) | C, blunt end addition of AA | |
| N2-dG-15-mer peptide cross-link | 5′-pAAACCGAGA-3′ | 18% | 939.36 (−3) | C |
| 5′-pAAACCGAGAA-3′ | 77% | 1043.82 (−3) | C, blunt end addition of A | |
| 5′-pAAACCGAGAAA-3′ | 5% | 1148.36 (−3) | C, blunt end addition of AA | |