Fig. 2.

Ablation of Fxr in the liver has a major impact on colonic gene expression.

Volcano plots of RNA sequencing data of colons of (A) Fxr-totKO, (B) Fxr-intKO, and (C) Fxr-livKO mice compared with controls. X-axes: genes located on the right-hand side of zero have a higher expression in the KO model compared with control, whereas points on the left of zero are more highly expressed in the control. Grey: |fold change| <2, q-value >0.05. Green: |fold change| >2, q-value >0.05. Blue: |fold change| <2, q-value <0.05. Red: |fold change| >2, q-value <0.05. The q-value is calculated by cufflink test statistics. (D) Proportional Venn diagram showing numbers of significantly changed genes in the colon of the different KO models compared with controls. Fxr, farnesoid X receptor; Fxr-intKO, intestine-specific Fxr knockout; Fxr-livKO, liver-specific Fxr knockout; Fxr-totKO, whole-body Fxr knockout; KO, knockout.