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. 2021 Jun 9;12(4):1297–1310. doi: 10.1016/j.jcmgh.2021.06.003

Figure 6.

Figure 6

FFAs activate YAP in human hepatocytes. (A) mRNA expression of ANKRD1, CYR61, CTGF, JUB, TGFB1, and WTIP in Huh7 and HepG2 cells treated with linoleic acid-oleic acid (FFAs) or palmitic acid (PA). Mean ± SD are graphed (n = 3 biological replicates; ∗P < .05, ∗∗P < .01). (B) Immunoblot of phosphorylated YAP, YAP, CYR61, and p38 MAPK in FFA-treated Huh7 cells. (C) YAP (yellow) and DAPI (blue) staining in Huh7 cells treated with FFAs. (D) mRNA expression of ANKRD1, CYR61, CTGF, and JUB in PHHs treated with FFAs. Mean ± SD are graphed (n = 3 biological replicates; ∗P < .05, ∗∗P < .01). (E) Immunoblot and densitometric analysis of phosphorylated YAP, YAP, and CYR61 in Huh7 cells treated with FFAs. Mean ± SD are graphed (n = 3 biological replicates; ∗∗P < .01).