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. 2021 Sep 13;118(38):e2101592118. doi: 10.1073/pnas.2101592118

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Fig. 1. — Cellular stress induces TRB3/USP9x-dependent Notch activation in KBP cells. (A) Immunoblot of USP9x, TRB3, MIB1, JAG1, and N1^IC in murine KBP cells transfected with either scrambled control (siSCR), TRB3, or USP9x siRNA and treated with either DMSO or 1 μM thapsigargin (Tg). (B) Immunoblot of USP9x, TRB3, MIB1, and N1^IC in KBP cells stably expressing a Dox-inducible, shRNA–targeting USP9x, without (−) or with (+) Dox and treated with either DMSO or Tg. (C) Immunoblot of USP9x, TRB3, MIB1, and N1^IC in KBP shUSP9x cells cultured in complete (+Leucine) or leucine-deprived (−Leucine) medium without and with Dox. Densitometry of the TRB3 and MIB1 protein bands are presented, relative to β-actin. The expression of β-actin or ERK1/2 are included as loading controls. Molecular weight markers are shown in kilodaltons.