Fig. 2.

PKA exerted as an indispensable factor for anti-inflammation of arctigenin in RAW264.7 cells. (A) Cells were treated with arctigenin for 15 min and collected for detection of phosphor-CREB by western blot. (B) Immunofluorescence of phospho-CREB in RAW264.7 cells. (C) Arctigenin, H89, and forskolin incubated RAW264.7 cells were analyzed for phosphor-CREB by western blot. (D) TNF-α level in RAW264.7 cells. (E) siPKA-transfected RAW264.7 cells were incuabted with arctigenin and/or forskolin, and then analyzed by western blotting. (F) TNF-α level in transfected RAW264.7 cells. All data were shown as means ± SEM (n = 3). *p < 0.05, **p < 0.01, and ***p < 0.001.