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. 2021 Mar 17;33:81–98. doi: 10.1016/j.jare.2021.03.006

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© 2021 The Authors. Published by Elsevier B.V. on behalf of Cairo University. This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

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Fig. 7 — Offspring production of functional oocytes from iGSC differentiation in vitro. (A) Mature oocytes from derived iGSCs after in vitro maturation. (B) The percentage of mature cumulus-oocyte complexes derived from iGSCs. Control, the immature oocytes from wild-type mice. (C) The percentage of fertilization of iGSC-derived MII oocytes. Control, MII oocytes from immature oocytes of wild type mice after in vitro maturation. (D) Two-cell embryos derived from iGSCs after in vitro fertilization. (E) Representative offspring derived from iGSC. (F) Number of offspring derived from iGSCs per litter. (G) Offspring were identified by Southern blotting. Lanes 1–4, offspring derived from iGSCs, lane W, wild-type mice. (H) Offspring were identified by fluorescence. Lanes 1–4, offspring derived from iGSCs, lane W, wild-type mice. Scale bars: 50 μm.