Figure 2.

Effects of miR-103-3p overexpression on NSC differentiation 96 hours after transduction.

(A) Map2 and Neurod1 expression were measured by quantitative polymerase chain reaction. The relative mRNA expression was normalized to miR-NC. (B) Flow cytometry quantification of Tuj1-positive cells after transfection. (C, D) Representative images and quantification of DCX- (green, stained with Alexa Fluor488) and MAP2- (green, stained with Alexa Fluor594) positive cells. The miR-103-3p group had a lower ratio of DCX- and MAP2-positive cells compared with the other groups. Scale bars: 100 μm. Data are expressed as the mean ± SD. Each point represents one independent experiment performed in duplicate. *P< 0.05, vs. miR-NC group (two-tailed paired Student's t-test). DCX: Doublecortin; MAP2: microtubule-associated protein 2; NSC: neural stem cell; Tuj1: class III beta-tubulin.