Figure 2.

MiR-29b increases glutamate-induced PC12 cell apoptosis.

PC12 cells were pretreated with miR-29b mimic, miR-29b inhibitor, and respective negative controls for 48 hours, followed by incubation with glutamate (12.5 mM) for 12 hours. (A, B) Flow cytometry detection of the percentage of PC12 cell apoptosis. All data are expressed as the mean ± SD. *P< 0.05 (Student's t-test). (C) Terminal deoxynucleotidyl transferase dUTP nick-end labeling assay of apoptotic PC12 cells (original magnification, 200×). The brightness of green fluorescence represents the degree of apoptosis. MiR-29b mimics induced apoptosis in PC12 cells, and miR-29b inhibitors decreased PC12 cell apoptosis. Each experiment was repeated three times. ctrl: Control; miR-29b: microRNA-29b.