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. 2021 Jul 8;17(2):433–439. doi: 10.4103/1673-5374.314319

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MiR-29b decreases SOD activity and increases MDA content in PC12 cells.

PC12 cells were pretreated with miR-29b mimic, miR-29b inhibitor, and the respective negative control for 48 hours, followed by incubation with glutamate (12.5 mM) for 12 hours. (A) MDA content in PC12 cells. (B) SOD activity in PC12 cells. (C) SOD-1 protein bands in PC12 cells, as determined by western blot assay. (D) Quantitative results of SOD-1 protein expression, which was normalized against β-actin. Values are represented as the mean ± SD. *P< 0.05 (Student's t-test). Each experiment was repeated three times. ctrl: Control; MDA: malondialdehyde; miR-29b: microRNA-29b; SOD: superoxide dismutase.