Table 1.

Characteristics of RT-qPCR master standard curves for four primer-probe sets.

Primer-probe sets⁎		Efficiency (E) (%)	Linearity (R2)	Slope	Y-intercept
CDC-N1	MC	99.7	0.982	−3.329	38.49
	SMC	265.1	0.727	−1.498	35.29
N-Sarbeco	MC	97.9	0.980	−3.374	40.50
	SMC	–	–	–	–
CCDC-N	MC	106.8	0.998	−3.169	39.07
	SMC	108.2	0.999	−3.139	40.39
E-Sarbeco	MC	93.2	0.999	−3.496	39.82
	SMC	153.4	0.995	−2.476	40.67

Note: *MC, Master calibration; SMC, Spiked mocks calibration.