Fig. 5.

SARS-COV-2 evoked antiviral innate and inflammatory response in hCECs. hCECs isolated from three human donor cadaver eyes were infected with SARS-CoV-2 parental strain USA-WA1/2020 (MOI of 1) for 24, 48, and 72h. qPCR analysis was performed for temporal expression of genes encoding TLRs (TLR 3, 4, 8, and 9), RIGI, IFNs (IFNα2, IFNβ1, and IFNλ1), IFN-induced antiviral response genes (ISG15, OAS2, and MX1), and inflammatory mediators (CCL5, CXCL10,IL6, IL1β, and TNFα). The experiment was performed in biological triplicates and statistical analysis was performed using one-way ANOVA; ns non-significant; *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001.