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. 2021 Aug 17;49(17):9755–9767. doi: 10.1093/nar/gkab706

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© The Author(s) 2021. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 4. — HDA6 can effectively deacetylate H3K9ac and H3K18ac in vitro. The hydrolysis of acetylated peptides by FLAG-tagged HDA6 was analyzed by LC–MS. Black traces show the total ion intensity for all ion species with m/z ranging from 300 to 2000; red traces show the ion intensity for the mass of unmodified (deacetylated) peptides; and blue traces show the ion intensity for the mass of acetylated peptides. TIC: total ion count.