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. 2021 Sep 3;49(17):9926–9937. doi: 10.1093/nar/gkab757

Figure 1.

Figure 1.

NgAgo belongs to a distinct clade of pAgos with a catalytic DEDX tetrad and novel repA domain. (A) Phyre 2 simulation 3D structure based on MjAgo structure (PDB: 5G5T). NgAgo structure is similar to MjAgo structure except for at the N-terminal domain. (B) Domain architecture analysis of NgAgo based on Phyre2 and HHpred reveals that NgAgo has an uncharacterized repA domain, a truncated N-terminal domain, a MID domain, and a PIWI domain. (C) Phylogenetic analysis of repA-containing pAgos (orange shaded) found from BLASTP against all isolates via JGI-IMG portal and other characterized pAgos. (D) The catalytic tetrad of NgAgo is conserved with catalytically active pAgos including MjAgo, PfAgo, MpAgo, and TtAgo in sequence alignment. (E) All residues of the catalytic tetrad (D663, E704, D738, and D863) DEDD, except E704 are structurally colocalized with the catalytic tetrad of MjAgo (D504, E541, D570 and D688).