Table 1.
Strains and plasmids
| Name | Relevant genotype | Vector backbone | Plasmid origin | Source |
|---|---|---|---|---|
| Strains | ||||
| BL21 (DE3) | F– ompT gal dcm lon hsdSB (rB–mB–) λ (DE3) [lacI lacUV5-T7p07 ind1 sam7 nin5]) [malB+]K-12 (λS) | (57) | ||
| MG1655 (DE3) | K-12 F– λ– ilvG– rfb-50 rph-1 (DE3) | (58) | ||
| MG1655 (DE3) atpI::KanR-mNeonGreen | K-12 F– λ– ilvG– rfb-50 rph-1 (DE3) atpI::KanR-mNeonGreen | This study | ||
| Plasmids | ||||
| pBSI-SceI (E/H) | bla | ColE1 derivative | (59) | |
| pTXTL-p70a-T7RNAP | Bla, P70-T7RNAP | unknown | Arbor Biosciences | |
| pET32a-GST-ELP64 | bla, lacI, PT7-GST-ELP64 | pBR322 | Professor Xin Ge (University of California, Riverside) | |
| pTKDP-hph | bla, hph, sacB | pMB1 | (32) | |
| pCas9-CR4 | cat, PTet-Cas9 | p15A | (60) | |
| pET-GST-Ago-His | bla, lacI, PT7-GST-NgAgo-His | pET32a-GST-ELP64 | pBR322 | This study |
| pET32a-His-Ago | bla, lacI, PT7-GST-NgAgo-His | pET32a-GST-ELP64 | pBR322 | This study |
| pET32a-His-repA | bla, lacI, PT7-His-repA | pET32a-GST-ELP64 | pBR322 | This study |
| pET-GST-N-del-His | bla, lacI, PT7-GST-N-del-His | pET32a-GST-ELP64 | pBR322 | This study |
| pET-GST-N-del/D663A/D738A-His | bla, lacI, PT7-GST- N-del/D663A/D738A -His | pET32a-GST-ELP64 | pBR322 | This study |
| pTKDP-KanR-mNeonGreen-hph | bla, hph, KanR-mNeonGreen | pTKDP-hph | pMB1 | This study |
| p15-KanR-PtetRed | cat, KanR-mNeonGreen, PTet-gam-beta-exo | pCas9-CR4 | p15A | This study |
| pET32-BFP | Amp, lacI, PT7-BFP | pET32a-GST-ELP64 and pBAD-mTagBFP2 | pBR322 | This study |
| pIncw-mNeonGreen | cat | pN565 (61) (origin of replication); pCas9-CR4 (60) (cat) | pIncW | This study |