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. 2021 Aug 20;49(17):10046–10060. doi: 10.1093/nar/gkab734

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© The Author(s) 2021. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial License (https://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com

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Figure 5. — Kinetic analysis of +1 frameshifting of SufB2 by analysis of fMP to fMPV and fMPR. (A) A kinetic scheme of rapid delivery of an equal mixture of SufB2-, Val- and Arg-TC to a 70SIC as described previously (10). (B) A kinetic scheme of rapid delivery of an equal mixture of Val- and Arg-TC over time to a stalled fMP-SufB2-POST complex as described previously (10). (C) A kinetic scheme of rapid delivery of an equal mixture of SufB2- and Val-TC to a 70SIC. The fractional conversion of fMP to fMPV was monitored over time, showing the yield and rate of conversion. (D) A kinetic scheme of rapid delivery of an equal mixture of SufB2- and Arg-TC to a 70SIC. The fractional conversion of fMP to fMPR was monitored over time, showing the yield and rate of conversion. (E) Summary of the fractional conversion of fMP to fMPV and fMPR from reactions (A), (B), (C) and (D) in bar graphs. The bar graphs in (A) and (B) were taken from previously published results (10) that were recalculated as the conversion from fMP to fMPV and fMPR. Each value in panels (C) and (D) represents mean ± SD, n = 3, of technical replicates, whereas values in panel (E) are means.