TABLE 3.

Synthetic lethalities between deletions in ccr4 and caf1 and mutations in the not genes^a

Construct	Growth
	wt	ccr4	caf1
wt	+	+	+
not1-2
30°C	+	+	+
34°C	+	−	−
pNOT1(1490–2108)
not1	+	Lethal	ND
not2	+	Lethal	Lethal
not2-1
30°C	+	+	ND
34°C	+	−	ND
not3	+	+	+
not3-2
30°C	+	+	ND
37°C	+	−	ND
not4	+	+	+
not5	+	Lethal	Lethal

^aGrowth was determined at 30°C on YEP medium supplemented with 2% glucose unless otherwise indicated. +, growth; −, no growth; lethal, the gene pair resulted in cell death; ND, not done. Isogenic strains used: wild type (wt), KY803; not1-2, MY8; not1-2 ccr4, MY8-1d; not1-2 caf1, MY8-c1c; not3, MY508; not3 caf1, MY508-c1b; not2-1, MY16; not2-1 ccr4, MY16-1c; not3, MY508; not3 caf1, MY508-c1b; not3-2, MY25; not3-2 ccr4, MY25-1b; not4, MY537-1; not4 ccr4, MY537-1-1b. not3 ccr4 segregants were obtained from 1076-2c-1 × MY508 crosses, and not4 caf1 segregants were obtained from 1278-5d × 1402-1a and 1278-5d × 1402-4a crosses. pNOT1(1480–2108) not1 ccr4 lethality was confirmed by analysis of segregants of diploid 1471 [1422-21/pNOT1(1480–2108)/pLexA-NOT1 × 1469-2-1c], ccr4 not2 lethality was confirmed from strain 1441 (188-1/pLexA-NOT2 × 1393-4a), ccr4 not5 lethality was confirmed from strain 1469 (1076-2c-1/pLexANOT5 × 1462-3a), caf1 not2 lethality was confirmed from strain 1442 (188-c1/pLexA-CAF1 × 1393-4a), and caf1 not5 lethality was confirmed from strain 1469 (1076-2c-1/pLexA-NOT5 × 1462-3c).