. Author manuscript; available in PMC: 2022 Oct 1.

Published in final edited form as: J Microbiol Methods. 2021 Aug 10;189:106300. doi: 10.1016/j.mimet.2021.106300

Table 1.

Myo-inositol levels in cell culture media and sera.

Sample name	Manufacturer and catalog number	Myo-inositol concentration (μM)
Sample name	Manufacturer and catalog number	Reported concentration	Yeast bioassay	(LC-MS/MS)
DMEM	GIBCO (31053028)	40	37.4 ± 8.13	36.50 ± 0.94
Inositol-free DMEM	MP Biomedicals (091642954)	0	BD^*	BD^*
Medium 199	GIBCO (11043023)	0.278	BD^*	0.34 ± 0.03
F.B.S.	HyClone, Cytiva (29489343)	-	29.18 ± 2.90	24.92 ± 0.82
Dialyzed F.B.S. ^**	HyClone, Cytiva (29489343)	-	B.D.^*	B.D.^*

BD: below detection (< 20 nM) for LC-MS/MS analysis) and (< 1 μM) for the yeast bioassay)

^**

Dialysis was performed against water using dialysis membrane tubing Spectra/Por 3 (Repligen) with a molecular weight cut-off (MWCO) of 3.5 kDa for 48 hours. The water in the dialysis container was replaced three times a day.