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. 2021 Sep 25;12(10):877. doi: 10.1038/s41419-021-04152-2

Fig. 3. Wnt5a and Wnt11 contributes to cardiac remodeling induced by pressure overload.

Fig. 3

A Western blot analysis of Wnt5a and Wnt11 in heart tissues (n = 6/group). B Cardiac fibrosis was analyzed by Masson staining in heart tissues (n = 5/group). C Western blot analysis of Col-1, MMP2, MMP9, and TGF-β1 levels in heart tissues (n = 5/group). Mice were injected with sh-Wnt5A /Wnt11-AAV9 (shWnt5a/11) or sh-Scramble-AAV9 (sh-NC) by tail vein, 2 weeks later, these mice were subjected to TAC or sham operation. After 4 weeks, further analysis was performed. D Western blot analysis of serum Wnt5a or Wnt11 level at 4 weeks after sham or TAC operation, and these mice were infused recombinant rat Wnt5a (rWnt5a) and Wnt11 (rWnt11), or vehicle (as control) by minipump for 2 weeks from the sham or TAC operation (n = 6/group). E Cardiac fibrosis was analyzed by Masson staining in heart tissues (n = 6/group). All data were shown as mean ± SEM; *P < 0.05, **P < 0.01, ***P < 0.001.