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. 2021 Sep 25;12(10):877. doi: 10.1038/s41419-021-04152-2

Fig. 4. Cardiomyocyte-secretory Wnt5a or Wnt11 is involved in pro-fibrotic effects under pressure overload.

Fig. 4

A, B Western analysis of Wnt5a or Wnt11 in stretched (MS) or control (Ctrl) cardiomyocytes pre-transfected sh-Scramble (sh-NC), sh-Wnt5a or sh-Wnt11 lentivirus, respectively. n = 5/group. C, D Western analysis of Col-1, MMP9, MMP2, and TGF-β1 in cardiac fibroblasts (CFs) treated with conditional medium from cardiomyocytes (CMs) in (A) or (B), respectively. n = 6/group Stretched (MS) or control (Ctrl) cardiomyocytes were pre-transfected with sh-Scramble (sh-NC), ()shWnt5a or shWnt11 lentivirus, respectively. The conditional medium of these cardiac myocytes was collected to treat fibroblasts for 24 h, and the expressions of Col-1, MMP9, MMP2, and TGF-β1 were detected by Western blot. All data were shown as mean ± SEM; *P < 0.05, **P < 0.01, ***P < 0.001.