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. 2021 Sep 25;12(10):877. doi: 10.1038/s41419-021-04152-2

Fig. 7. Inhibition of EGFR activation attenuates the pro-fibrotic effect induced by Wnt5a or Wnt11 in neonatal rat cardiac fibroblasts.

Fig. 7

A, B The expression of EGFR, Col-1, α-SMA, MMP9, MMP2, and TGF- β1 were detected by western blot in cardiac fibroblasts. After transfection with si-RNA-EGFR (si-EGFR) or si-RNA-scramble (si-Scram) for 48 h, these fibroblasts were treated with recombinant rat Wnt5a (rWnt5a) or Wnt11 (rWnt11) for 24 h, respectively. The same volume of PBS was treated as a control. n = 6/group. C, D The levels of p-ERK, p-P38, FZD5, and EGFR were examined by western blot analysis in cardiac fibroblasts. These fibroblasts were transfected with si-RNA-EGFR (si-EGFR) or si-RNA-scramble (si-Scram) for 48 h and then treated with recombinant rat Wnt5a (rWnt5a) or Wnt11 (rWnt11), respectively, 30 min later, these cells were analyzed. n = 5/group. All data were shown as mean ± SEM; *P < 0.05, **P < 0.01, ***P < 0.001.