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. 2021 Sep 25;12(10):877. doi: 10.1038/s41419-021-04152-2

Fig. 8. SiRNA-FZD5 attenuates the activation of neonatal rat cardiac fibroblasts induced by Wnt5a or Wnt11.

Fig. 8

A, B Western blot analysis of FZD5, Col-1, α-SMA, MMP9, MMP2, and TGF-β1 expression in cardiac fibroblasts. Si-RNA-FZD5 (si-FZD5) or si-RNA-scramble (si-Scram) was treated with fibroblasts for 48 h, then exogenous recombinant rat Wnt5a (rWnt5a) or Wnt11 (rWnt11) were added into cultured fibroblasts for 24 h for further analysis. n = 6/group. C, D Western blot analysis of p-EGFR, EGFR, p-ERK, p-P38, and FZD5 levels in cardiac fibroblasts. Si-RNA-FZD5 (si-FZD5) or si-RNA-scramble (si-Scram) was treated into fibroblasts for 48 h, then exogenous recombinant rat Wnt5a (rWnt5a) or Wnt11 (rWnt11) were added into cultured fibroblasts for 30 min for further analysis. n = 5/group. All data were shown as mean ± SEM; *P < 0.05, **P < 0.01, ***P < 0.001.