Fig. 4. HAT1 stabilizes EZH2 by competing with UBR4 for binding to EZH2.

a,b PANC-1 cells were infected with lentivirus expressing control, HAT1-specific shRNAs, UBR4-specific shRNAs, and HAT1-overexpressing plasmid. After infecting 72 h, cells were harvested for western blotting analysis (n = 3). c PANC-1 cells were infected with indicated plasmids. After 72 h infection, cells were treated with Cycloheximide (CHX) and then collected for western blotting analysis at different timepoints. d, e PANC-1 cells were infected with indicated plasmids. After 48 h, PANC-1 cells were infected again with HA-Ub plasmid for 24 h and treated with MG132 (10 μM) for another 8 h before harvesting cells. Western Blotting analysis was conducted after co-immunoprecipitation.