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. 2021 Sep 25;12(10):878. doi: 10.1038/s41419-021-04118-4

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 5 — a The scheme for CS-TPP-siHAT1 preparation. 250 ug siHAT1 was added into 1 mg tripolyphosphate (TPP) solution. Then, the above mixture solution was added into 5 mg chitosan (CS) solution dropwise and stirring slowly for 30 min to prepare the CS-TPP-siHAT1.TPP tripolyphosphate, CS chitosan. b Different magnifications TEM images of CS-TPP-siHAT1. c The cellular uptake confocal images of CS-TPP-siRNA in PANC-1, BxPC-3, and MIA-PaCa cells. d PANC-1, BxPC-3, and MIA-PaCa cells were treated with different CS-TPP-siHAT1 concentration (10 μM, 20 μM, 50 μM) for 3 days. The cells were harvested to extract total protein and RNA respectively, and then RT-qPCR and western blotting were performed to analyzed HAT1, PVT1, and EZH2 expression level. All data were shown as mean values ± SD (n = 3). *P < 0.05; **P < 0.01; ***P < 0.001. e PANC-1, BxPC-3, and MIA-PaCa cells were treated with 20 μM CS-TPP-siHAT1 for different time (0 days, 1 days, 2 days, 3 days). The cells were harvested to extract total protein and RNA, respectively, and then RT-qPCR and western blotting were performed to analyzed HAT1, PVT1, and EZH2 expression level. All data were shown as mean values ± SD (n = 3). *P < 0.05; **P < 0.01; ***P < 0.001.